흰쥐 폰에브너씨 샘 및 뒤혀샘에서의 히할루론산 수용체(CD44)와 복합당질 분포에 관한 연구
Localization of CD44(the hyaluronate receptor) and Glycoconjugates on the von Ebner's Lingual Gland and Posterior Lingual Gland in the Adult Rat
소속 상세정보
1박봉수/1B.S. Park
1한상욱/1김규천/2박혜련/3윤식/3유기수/3유영현/1S.U. Han/1K.C. Kim/2H.R. Park/3S. Yoon/3K.S. Yoo/3Y.H. Yoo
KMID : 0355819960200020319
Abstract
-Abstract-
Von Ebner's lingual glands are located under the vallate and the folliate papillae and
secrete serous materials. Posterior lingual glands are also located under vallate papilla on
the dorsal posterior third of the tongue and secrete mucous materials. The hyaluronate
receptor(CD44) is a cell surface glycoprotein of 85kD as a mulitifunctional adhesion
molecule that binds hyaluronic acid, type I collagen, and fibronectin. Lectins are
sugar-binding proteins or glycoproteins of non-immune origin which agglutinate cells
and/or precipitate complex carbohydrates.
Therefore, the aim of the present study is to investigate distribution of the CD44 and
glycoconjugates in the von Ebner's gland and posterior lingual gland of the rat tongue.
The CD44 and glycoconjugates in the von Ebner's gland and posterior lingual gland
were studied by means of immunohistochemical techniques using mouse anti-rat CD44
antibody and 15 kinds of lectins(LCA, PNA, VVL, UEA-I, PHA-L, PHA-E, PSA, SJA,
RCA, DSL, DBA, BSL-I, SBA, WGA, Con-A) respectively. CD44 is localized on the
wholly plasma membrances and cytoplasms in the serous acinar cells and serous
demilune. And CD44 is mainly localized on the basal and basolateral plasma membrance
of the mucous acinar cells. In the plasma membrance of ductal cells CD44 is focally
localized on the von Ebner's lingual gland were positively reacted with LCA, PNA,
VVL, PHA-E, RCA, DSL, DBA, BSL-I, SBA, WGA and Con-A. And the mucous acinar
cells on the posterior lingual gland were stained with LCA, VVL, PSA, RCA, DSL,
DBA, B니-I, SBA and WGA. The cells of the serous demilune on the lingual posterior
gland were positively rected with LCA, PHA-E, PSA, RCA, DSL, DBA, BSL-I, SBA,
WGA and Con-A. The ductal cells on the von Ebner's lingual gland were positively
reacted with LCA, PHA-L, DBA, WGA and Con-A. And the ductal cells on the
posterior lingual glands were stained with LCA, VVL, PHA-L, RCA, DSL, DBA and
WGA.
The function of CD44 on the plasma membrance in the epithelium is not yet known.
The difference in the positive immunereactivity on the serous acinar cells and mucous
acinar cells was strikingly interesting results. In the results of lectin histochemistry,
generally serous acinar cells have staining capacities for applied lectins, whereas mucous
acinar cells do not bind or bind relatively weakly for applied lectins.
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